anti tap1 primary antibody  (Proteintech)

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Machine learning-based screening of candidate genes for DKD. ( A-B ) LASSO regression analysis identified 5 candidate genes. ( C-D ) Random forest analysis selected 10 top-ranked genes based on relative importance. ( E ) Venn diagram of the overlapping hub genes (CASP1, PRKX, TAP1) derived from LASSO regression and random forest analysis. (F) Protein-protein interaction network of hub genes generated by GeneMANIA

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Derivative Assay, Generated

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Validation of hub gene (CASP1, PRKX, TAP1) expression patterns in DKD. ( A ) Scatter plots comparing expression levels between DKD patients and controls in the GSE30122 dataset. ( B ) Heatmap of normalized expression for the three hub genes across samples. ( C-E ) Independent validation in renal tubule tissues using Nephroseq v5 database: ( C ) CASP1, ( D ) PRKX, and ( E ) TAP1. ( F-H ) Correlation of hub gene expression with glomerular filtration rate: ( F ) CASP1, ( G ) PRKX, and ( H ) TAP1. Statistical analysis: t-tests for normally distributed data (Fig. 6A, C) and Wilcoxon rank-sum tests for non-normal data (Fig. 6D, E). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, no significance

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Biomarker Discovery, Expressing, Gene Expression, Filtration

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: GSEA of hub genes using GO and KEGG gene sets from MSigDB datasets. ( A-B ) GSEA results for CASP1: ( A ) GO and ( B ) KEGG analyses. ( C-D ) GSEA results for PRKX: ( C ) GO and ( D ) KEGG analyses. ( E-F ) GSEA results for TAP1: ( E ) GO and ( F ) KEGG analyses

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques:

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Immune infiltration analysis in DKD. ( A ) Box plot showing the relative abundance of 22 immune cell types across different groups. ( B-D ) correlation analysis between the expression of hub genes and infiltrating immune cell proportions: ( B ) PRKX, ( C ) TAP1, and ( D ) CASP1. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, no significance

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Expressing

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Single-cell RNA sequencing profile of kidney tissues. ( A ) UMAP projection after Harmony batch correction, integrating datasets across samples. ( B ) 22 distinct clusters visualized by UMAP plotting. ( C ) Annotation of 10 major cell types based on marker genes. ( D ) Dot plot illustrating the expression of marker genes in different cell types. ( E-G ) Bubble plots comparing expression patterns of three hub genes in PT cells (DKD vs. controls). ( E ) CASP1, ( F ) TAP1, and ( G ) PRKX. Statistical significance was determined by Wilcoxon rank-sum tests (**** P < 0.0001). LOH, loop of Henle cells; PT, proximal tubule cells; DCT, distal convoluted tubule cells; ENDO, endothelial cells; FIB, fibroblasts; LEUK, leukocytes; ICA, type A intercalated cells; PODO, podocytes; PEC, parietal epithelial cells; ICB, type B intercalated cells

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Single Cell, RNA Sequencing, Marker, Expressing

Journal: Inflammation

Article Title: Integrated Transcriptomic Analysis Identifies TAP1 as a Key Regulator of PANoptosis in Diabetic Kidney Disease Tubular Injury

doi: 10.1007/s10753-025-02400-7

Figure Lengend Snippet: Validation of hub genes in experimental and clinical DKD. ( A ) qRT-PCR analysis of TAP1, CASP1, and PRKX expression in HK-2 cells under low glucose (LG, 5.5 mmol/L glucose) or high glucose (HG, 25 mmol/L glucose) conditions. ( B ) Western blot analysis of TAP1 protein levels in LG or HG. ( C ) Representative kidney histology (H&E, PAS, and Masson trichrome staining) in db/m and db/db mice. Scale bar, 20 μm. ( D ) Western blot of TAP1 protein levels in kidney tissues from db/m and db/db mice ( n = 3 for each group). ( E ) Representative IHC staining of TAP1 in db/m ( n = 3) and db/db ( n = 3) mouse kidney tissue. Scale bar, 50 μm. ( F ) Representative IHC staining of TAP1 in human kidney tissues from DKD ( n = 3) and control ( n = 3) patients. Scale bar, 50 μm. * P < 0.05, ** P < 0.01; ns, no significance

Article Snippet: Paraffin-embedded kidney sections were subjected to antigen retrieval, followed by overnight incubation at 4 °C with anti-TAP1 primary antibody (1:200, Proteintech).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Western Blot, Staining, Immunohistochemistry, Control